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The moss genes PpSKI1 and PpSKI2 encode nuclear SnRK1 interacting proteins with homologues in vascular plants
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2007 (English)In: Plant Molecular Biology, ISSN 0167-4412, E-ISSN 1573-5028, Vol. 64, no 5, 559-573 p.Article in journal (Refereed) Published
Abstract [en]

The yeast Snf1, animal AMPK, and plant SnRK1 protein kinases constitute a family of related proteins that have been proposed to serve as metabolic sensors of the eukaryotic cell. We have previously reported the characterization of two redundant SnRK1 encoding genes (PpSNF1a and PpSNF1b) in the moss Physcomitrella patens. Phenotypic analysis of the snf1a snf1b double knockout mutant suggested that SnRK1 is important for the plant’s ability to recognize and adapt to conditions of limited energy supply, and also suggested a possible role of SnRK1 in the control of plant development. We have now used a yeast two-hybrid system to screen for PpSnf1a interacting proteins. Two new moss genes were found, PpSKI1 and PpSKI2, which encode highly similar proteins with homologues in vascular plants. Fusions of the two encoded proteins to the green fluorescent protein localize to the nucleus. Knockout mutants for either gene have an excess of gametophores under low light conditions, and exhibit reduced gametophore stem lengths. Possible functions of the new proteins and their connection to the SnRK1 kinase are discussed.

Place, publisher, year, edition, pages
2007. Vol. 64, no 5, 559-573 p.
Keyword [en]
AMPK, Gametophores, Physcomitrella patens, Plant Development, Snf1, SnRK1
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-15851DOI: 10.1007/s11103-007-9176-5ISI: 000247185300007PubMedID: 17533513OAI: oai:DiVA.org:uu-15851DiVA: diva2:43622
Available from: 2008-03-11 Created: 2008-03-11 Last updated: 2017-12-08Bibliographically approved
In thesis
1. Studies of the Carbon and Energy Metabolism in the Moss Physcomitrella patens
Open this publication in new window or tab >>Studies of the Carbon and Energy Metabolism in the Moss Physcomitrella patens
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Since a proper balance between anabolic and catabolic reactions is essential for all eukaryotes, the basic mechanisms for regulation of the energy and carbon metabolism have been conserved throughout evolution. The moss Physcomitrella patens, which belongs to one of the basal clades among land plants, has many unique properties that make it an excellent plant model system.

We have used a yeast two-hybrid system to identify novel possible regulators or targets of the moss Snf1-related kinases, previously shown to regulate energy homeostasis. The function of the identified interactors PpSki1 and PpSki2 was analyzed in order to better understand the biological role of plant Snf1-related kinases.

The recently completed genome sequence of Physcomitrella was used in a comparative approach to study to what extent key enzyme and gene families involved in transport and metabolism of sugars and in regulation of the energy and carbon metabolism are conserved between mosses and vascular plants.

It has long been known that transformed DNA can replicate episomally in Physcomitrella. We have now shown that such DNA can be rescued back into E. coli. Surprisingly, we found that the original plasmid can be recovered from moss transformants obtained with circular DNA. Plasmids rescued from transformants obtained with linearized DNA had been repaired either by homologous recombination or by cohesive end re-ligation. These findings suggest that methods using shuttle plasmids are feasible in Physcomitrella.

Hexokinase, a key enzyme in the carbon metabolism, catalyzes the first step in hexose metabolism, but is also involved in sugar sensing and signaling. We have now made an initial characterization of the complete hexokinase family in Physcomitrella which is encoded by 11 genes. Two new types of plant hexokinases, types C and D, were found in addition to the previously described types A and B.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 73 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, ISSN 1651-6206 ; 490
Keyword
Hexokinase, SnRK1, sugar signaling, carbon metabolism, Physcomitrella patens, shuttle vector, subcellular localization, model organism
National Category
Cell and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:uu:diva-109358 (URN)978-91-554-7631-1 (ISBN)
Public defence
2009-11-27, B41, Uppsala Biomedical Center (BMC), Husargatan 3, 75123 Uppsala, 09:15 (English)
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Available from: 2009-11-05 Created: 2009-10-14 Last updated: 2009-11-05Bibliographically approved

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Nilsson, AndersJohansson, MonikaRonne, Hans

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