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Inhibition of HPV-16 L1 expression from L1 cDNAs correlates with the presence of hnRNP A1 binding sites in the L1 coding region
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2008 (English)In: Virus genes, ISSN 0920-8569, E-ISSN 1572-994X, Vol. 36, no 1, 45-53 p.Article in journal (Refereed) Published
Abstract [en]

The human papillomavirus type 16 (HPV-16) L1 capsid protein is very poorly expressed from cDNA expression plasmids transiently transfected into mammalian cells. The results described herein demonstrate that inhibition of HPV-16 L1 expression from L1 cDNAs correlates with the presence of splicing regulatory sequences in the L1 coding region. This inhibitory effect correlates with the binding of hnRNP A1 to the RNA elements. Similar to unutilised splice sites that may retain mRNAs in the nucleus, regulatory splicing RNA elements may also inhibit gene expression in the absence of splicing. The results presented here explain the inefficient expression of HPV-16 L1 protein from the wild type L1 cDNA expression plasmids in mammalian cells. These results may be of general interest since alteration of RNA sequences to prevent unwanted RNA-protein interactions may increase expression of many different genes in transient transfections or after plasmid uptake in DNA vaccination approaches.

Place, publisher, year, edition, pages
2008. Vol. 36, no 1, 45-53 p.
Keyword [en]
HPV-16, human papillomavirus, splicing, hnRNP A1, L1, L2, silencer, late genes, RNA, RNA processing
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-15860DOI: 10.1007/s11262-007-0174-0ISI: 000252890200006PubMedID: 18040766OAI: oai:DiVA.org:uu-15860DiVA: diva2:43631
Available from: 2008-03-11 Created: 2008-03-11 Last updated: 2017-12-08Bibliographically approved

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Schwartz, Stefan

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