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QSAR of multiple mutated antibodies
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences, Pharmaceutical Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences, Pharmaceutical Pharmacology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences, Pharmaceutical Pharmacology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
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2007 (English)In: Journal of Molecular Recognition, ISSN 0952-3499, E-ISSN 1099-1352, Vol. 20, no 2, 97-102 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to develop predictive quantitative structure-activity relationship (QSAR) modeling for antibody-peptide interactions. A small single chain antibody library was designed and manufactured around the murine anti-p24 (HIV-1) monoclonal antibody CB4-1 by use of statistical molecular design (SMD) principles and site directed mutagenesis, and its affinity for a p24 derived antigen was determined by fluorescence polarization. A satisfactory QSAR model (Q2 = 0.74, R2 = 0.88) was derived by correlating the affinity data to physicochemical property scales of the amino acids varied in the library. The model explains most of the antibody-antigen interactions of the studied set, and provides insights into the molecular mechanism involved in antigen binding.

Place, publisher, year, edition, pages
2007. Vol. 20, no 2, 97-102 p.
Keyword [en]
Amino acid physicochemical properties, Antibody library, Interaction site mapping, Peptide epitope, QSAR, Single chain antibody, Site directed mutagenesis, Statistical molecular design
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-15889DOI: 10.1002/jmr.817ISI: 000246169000004PubMedID: 17421049OAI: oai:DiVA.org:uu-15889DiVA: diva2:43660
Available from: 2008-06-05 Created: 2008-06-05 Last updated: 2017-12-08Bibliographically approved

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Yahorava, Sviatlana

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Pharmaceutical PharmacologyDepartment of Cell and Molecular Biology
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