Pressurized solvent extraction and monolithic column-HPLC/DAD analysis of anthocyanins in red cabbage
2008 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, Vol. 74, no 5, 1218-1223 p.Article in journal (Refereed) Published
The aim of this work was to develop a fast method for extraction and analysis of anthocyanins in red cabbage. Pressurized hot water containing 5% of ethanol was used as an extremely efficient extraction solvent. HPLC/DAD with a monolithic column was used to accomplish a fast analysis-24 anthocyanin peaks within 18 min. Statistical design was used to optimize the studied extraction parameters: temperature (80-120 degrees C); sample amount (1-3 g); extraction time (6-11 min); concentration of formic acid in the extraction solvent (0-5 vol.%). The best extraction conditions for a majority of the anthocyanin peaks were 2.5 g of sample, 99 degrees C (at 50 bar), 7 min of extraction and a solvent composition of water/ethanol/formic acid (94/5/1, v/v/v).
Place, publisher, year, edition, pages
2008. Vol. 74, no 5, 1218-1223 p.
Anthocyanins, Red cabbage, Monolithic column, Accelerated solvent extraction, Polyphenols
IdentifiersURN: urn:nbn:se:uu:diva-15967DOI: 10.1016/j.talanta.2007.08.029ISI: 000253514700018OAI: oai:DiVA.org:uu-15967DiVA: diva2:43738