uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
The enigma of in vivo oxidative stress assessment: isoprostanes as an emerging target
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences. (Clinical Nutrition)
2007 (English)In: Scandinavian Journal of Food & Nutrition, ISSN 1748-2984 (electronic) 1748-2976 (paper), Vol. 51, no 2, 48-61 p.Article in journal (Refereed) Published
Abstract [en]

Oxidative stress is believed to be one of the major factors behind several acute and chronic diseases, and may also be associated with ageing. Excess formation of free radicals in miscellaneous body environment may originate from endogenous response to cell injury, but also from exposure to a number of exogenous toxins. When the antioxidant defence system is overwhelmed, this leads to cell damage. However, the measurement of free radicals or their endproducts is tricky, since these compounds are reactive and short lived, and have diverse characteristics. Specific evidence for the involvement of free radicals in pathological situations has been difficult to obtain, partly owing to shortcomings in earlier described methods for the measurement of oxidative stress. Isoprostanes, which are prostaglandin-like bioactive compounds synthesized in vivo from oxidation of arachidonic acid, independently of cyclooxygenases, are involved in many human diseases, and their measurement therefore offers a way to assess oxidative stress. Elevated levels of F2-isoprostanes have also been seen in the normal human pregnancy, but their physiological role has not yet been defined. Large amounts of bioactive F2-isoprostanes are excreted in the urine in normal basal situations, with a wide interindividual variation. Their exact role in the regulation of normal physiological functions, however, needs to be explored further. Current understanding suggests that measurement of F2-isoprostanes in body fluids provides a reliable analytical tool to study oxidative stress-related diseases and experimental inflammatory conditions, and also in the evaluation of various dietary antioxidants, as well as drugs with radical-scavenging properties. However, assessment of isoprostanes in plasma or urine does not necessarily reflect any specific tissue damage, nor does it provide information on the oxidation of lipids other than arachidonic acid.

Place, publisher, year, edition, pages
2007. Vol. 51, no 2, 48-61 p.
Keyword [en]
antioxidants, free radicals, human, inflammation, isoprostanes, oxidative strain, oxidative stress
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-16050DOI: 10.1080/17482970701411642OAI: oai:DiVA.org:uu-16050DiVA: diva2:43821
Available from: 2008-04-11 Created: 2008-04-11 Last updated: 2010-03-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Basu, Samar
By organisation
Department of Public Health and Caring Sciences
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 189 hits
ReferencesLink to record
Permanent link

Direct link