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Hormonal regulation of renomedullary hyaluronan
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrativ Fysiologi.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Transplantation Surgery.
Department of Medicine, Georgetown University Medical Centre, Washington, DC, USA.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2008 (English)In: Acta Physiologica, ISSN 1748-1708, E-ISSN 1748-1716, Vol. 193, no 2, 191-198 p.Article in journal (Refereed) Published
Abstract [en]

AIM

Hyaluronan (HA) is involved in renomedullary water handling through its water-binding capacity. This study addressed the effect of hormones involved in regulating fluid-electrolyte homeostasis on renomedullary HA content in vivo and in vitro.

METHODS

The kidneys from rats treated with L-NAME, indomethacin, vasopressin (AVP) or methylprednisolone (MP) during euvolaemia or water loading were analysed for HA by RIA, ELISA and histochemical staining. HA was measured in renomedullary interstitial cells treated with AVP, angiotensin II (Ang II) or a combination of AVP and Ang II.

RESULTS

 Baseline renal cortical and medullary HA content was unaffected by 2 h of intravenous treatment with L-NAME (NOS inhibitor) or indomethacin (cyclo-oxygenase inhibitor), whereas AVP reduced medullary HA by 33%. During 2 h of acute water loading, diuresis was accompanied by an increase in renomedullary HA (+45%), but cortical HA was unaffected. In both L-NAME- and indomethacin-treated animals, the water loading-induced increase in renomedullary HA was absent, indicating involvement of NO and prostaglandins. After 7 days of MP treatment, medullary HA was reduced by 40%, but the water loading-induced elevation in HA remained. In cultured renomedullary interstitial cells, AVP reduced the HA content in the supernatant by 63%, and simultaneous treatment with Ang II reduced the HA content even further (95%).

CONCLUSION

 AVP reduces HA content, and NO and prostaglandins are needed for the increase in HA during water loading.

Place, publisher, year, edition, pages
2008. Vol. 193, no 2, 191-198 p.
Keyword [en]
hyaluronan, kidney, nitric oxide, prostaglandins, vasopressin, water loading
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-16169DOI: 10.1111/j.1748-1716.2007.01795.xISI: 000255601300009PubMedID: 18081884OAI: oai:DiVA.org:uu-16169DiVA: diva2:43940
Available from: 2008-05-08 Created: 2008-05-08 Last updated: 2017-12-08Bibliographically approved

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Rügheimer, LouiseJohnsson, C.Hansell, Peter

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