Simultaneous analysis of five antidepressant drugs using direct injection of biofluids in a capillary restricted-access media-liquid chromatography-tandem mass spectrometry system
2008 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1189, no 1-2, 514-522 p.Article in journal (Refereed) Published
Direct analysis, with minimal sample pretreatment, of antidepressant drugs, fluoxetine, imipramine, desipramine, amitriptyline, and nortriptyline in biofluids was developed with a total run time of 8 min. The setup consists of two HPLC pumps, injection valve, capillary RAM-ADS-C18 pre-column and a capillary analytical C 18 column connected by means of a six-port valve in backflush mode. Detection was performed with ESI-MS/MS and only 1 mu m of sample was injected. Validation was adequately carried out using FLU-d(5) as internal standard. Calibration curves were constructed under a linear range of 1-250 ng mL(-1) in plasma, being the limit of quantification (LOQ), determined as 1 ng mL(-1), for all the analytes. With the described approach it was possible to reach a quantified mass sensitivity of 0.3 pg for each analyte (equivalent to 1.1-1.3 fmol), translating to a lower sample consumption (in the order of 103 less sample than using conventional methods).
Place, publisher, year, edition, pages
2008. Vol. 1189, no 1-2, 514-522 p.
Column switching, Restricted-access media (RAM), Capillary liquid chromatography, Tandem mass spectrometry, Antidepressant drugs, Sample preparation automation
IdentifiersURN: urn:nbn:se:uu:diva-16216DOI: 10.1016/j.chroma.2008.02.107ISI: 000255698000054OAI: oai:DiVA.org:uu-16216DiVA: diva2:43987