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Laminin deposition is dispensable for vasculogenesis but regulates blood vessel diameter independent of flow
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2008 (English)In: The FASEB Journal, ISSN 0892-6638, Vol. 22, no 5, 1530-1539 p.Article in journal (Refereed) Published
Abstract [en]

Basement membranes (BMs) consisting of laminins, collagens, and heparan sulfate proteoglycans (HSPGs) are vital for proper endothelial cell function, but many aspects of their role in vascular development remain unknown. Here, we demonstrate that vascular structures within differentiating embryoid bodies are wrapped in a BM composed of alpha 4- and alpha 5-chain laminins, fibronectin, collagen IV, and HSPGs. In sprouting angiogenesis, laminins were produced by stalk cells, as well as the leading tip cell, and deposited along the sprout length, including tip cell filopodia. In embryonic stem cells deficient in laminins, due to lamc1 (laminin gamma 1) deletion, vascular development and organization were largely unaffected. However, the frequency of vessels with wide lumens was increased 4-fold. Laminin-deficient vessels were moreover characterized by increased fibronectin levels and enhanced endothelial cell proliferation. We conclude that laminins are dispensable for vascular development but that they regulate lumen formation in the absence of flow and vascular tone.

Place, publisher, year, edition, pages
2008. Vol. 22, no 5, 1530-1539 p.
Keyword [en]
angiogenesis, sprouting, basement membrane, VEGF
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-16228DOI: 10.1096/fj.07-9617comISI: 000255898700025PubMedID: 18073332OAI: oai:DiVA.org:uu-16228DiVA: diva2:43999
Available from: 2008-05-13 Created: 2008-05-13 Last updated: 2009-11-04Bibliographically approved

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Claesson-Welsh, Lena
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