In situ cross-linkable hyaluronan hydrogel enhances chondrogenesis
2011 (English)In: Journal of tissue engineering and regenerative medicine, ISSN 1932-6254, Vol. 5, no 8, E188-E196 p.Article in journal (Refereed) Published
The present work describes the feasibility of a cross-linkable injectable hyaluronan hydrogel for cartilage repair. The hydrogel used is a two-component system based on aldehyde-modified hyaluronan and hydrazide-modified polyvinyl alcohol, which are rapidly cross-linked in situ upon mixing. The in vitro study showed that chondrocytes and mesenchymal cells cultured in the gel form cartilage-like tissue, rich in glycosaminoglycans, collagen type II and aggrecan. In a rabbit animal model the injection of the hydrogel improved the healing of a full-thickness cartilage defect created in the knee as compared to non-treated controls. This rabbit study showed that the regenerated cartilage defects stained more intensely for type II collagen upon treatment with the hydrogel. The hyaluronan-based hydrogel may be used as a delivery vehicle for both growth factors and/or cells for cartilage repair. The in vivo study also indicated that the hydrogel alone has a beneficial effect on cartilage regeneration.
Place, publisher, year, edition, pages
2011. Vol. 5, no 8, E188-E196 p.
cartilage, injectable hyaluronan, cross-linked, bone morphogenetic protein-2, rabbit model
Natural Sciences Polymer Chemistry
Research subject Chemistry with specialization in Polymer Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-158604DOI: 10.1002/term.415ISI: 000294164500005OAI: oai:DiVA.org:uu-158604DiVA: diva2:440207