uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Analysis of short DNA fragments from Holocene peatmoss samples
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Ecological Botany.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Evolutionary Functional Genomics.
2008 (English)In: The Holocene, ISSN 0959-6836, E-ISSN 1477-0911, Vol. 18, no 6, 1003-1006 p.Article in journal (Refereed) Published
Abstract [en]

This paper describes our recent attempt to isolate and analyse DNA from old plant remains of the common peatmoss Sphagnum fuscum retrieved from a peat core collected in the mire Fuglmyra, in central Norway. DNA was recoverable and usable from sub-fossilized (10–450 years old) plant remains of the peatmoss. A chloroplast (trnL) and two nuclear (ITS2 and RAPDf) regions were co-amplified from 80 samples of different ages. The RAPDf region was the only variable one with three different haplotypes found among five samples. Comparison of the ancient sequences with modern sequences found in the extant population occurring at the same site ascertained a genetic link between modern and fossil samples of this species. This retrieval of ancient DNA from sub-fossilized moss remains isolated from peat cores has important implications for the palaeoecology of peatmosses by allowing direct estimates of plant population dynamics in space and time.

Place, publisher, year, edition, pages
2008. Vol. 18, no 6, 1003-1006 p.
Keyword [en]
Ancient DNA, Holocene, ITS2, Norway, Palaeoecology, Peatlands, Population dynamics, RAPDf, Sphagnum, trnL
National Category
Research subject
Ecological Botany
URN: urn:nbn:se:uu:diva-16260DOI: 10.1177/0959683608093540ISI: 000258754900013OAI: oai:DiVA.org:uu-16260DiVA: diva2:44031
Available from: 2008-08-05 Created: 2008-08-05 Last updated: 2012-11-08

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Gunnarsson, UrbanParducci, Laura
By organisation
Ecological BotanyEvolutionary Functional Genomics
In the same journal
The Holocene

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 261 hits
ReferencesLink to record
Permanent link

Direct link