Unfolding proximity ligation probes for measuring and imaging individual nucleic acid and protein molecules
(English)Manuscript (preprint) (Other academic)
We present a new class of probes for molecular detection reactions - probes for unfolding proximity ligation assays. These are secondarily structured nucleic acid reagents that can be induced to unfold in order to undergo proximity ligation reactions, followed by visualization via localized single molecule amplification. The probes enable highly specific targeting of individual nucleic acid or protein molecule. We demonstrated the performance of these new probes by detecting synthetic DNA and cancer-related transcripts in situ and multiplex probing of proteins in solution.
unfolding, proximity ligation, single molecule, protein biomarker, cancer-related transcript
Biomedical Laboratory Science/Technology
Research subject Molecular Medicine
IdentifiersURN: urn:nbn:se:uu:diva-158635OAI: oai:DiVA.org:uu-158635DiVA: diva2:441193