Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays
2008 (English)In: Cancer Letters, ISSN 0304-3835, Vol. 265, no 1, 98-106 p.Article in journal (Refereed) Published
Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan (TM) antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naive B cells.
Place, publisher, year, edition, pages
2008. Vol. 265, no 1, 98-106 p.
CD antigens, antibody microarrays, DNA microarrays, B cell sub-populations, lymphoma
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-16383DOI: 10.1016/j.canlet.2008.02.006ISI: 000256730200010PubMedID: 18353541OAI: oai:DiVA.org:uu-16383DiVA: diva2:44154