Detection of rolling circle amplified DNA molecules using probe-tagged magnetic nanobeads in a portable AC susceptometer
2011 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 29, no 1, 195-199 p.Article in journal (Refereed) Published
Here, the volume-amplified magnetic nanobead detection assay (VAM-NDA) is for the first time applied for detection of rolling circle amplified (RCA) DNA molecules in a portable, commercial AC susceptometer that operates at ambient temperatures and with an analysis time of about 20 min. The performance of the assay is investigated using three different magnetic nanobead sizes: 50, 130 and 250 nm. The performance of the assay using the AC susceptometer is compared to the performance achieved using a superconducting quantum interference device (SQUID).
It is found that the performance of the assay is comparable in the two setups with a quantitative detection limit of ∼4 pM for all bead sizes under study.
The findings show that the VAM-NDA holds promise for future wide-spread implementation in commercial AC susceptometer setups thus opening up for the possibility to perform magnetic bead-based DNA detection in point-of-care and outpatient settings.
Place, publisher, year, edition, pages
2011. Vol. 29, no 1, 195-199 p.
Probe-tagged magnetic beads; Padlock probes; Rolling circle amplification; Brownian relaxation; AC susceptometer
Nano Technology Engineering and Technology
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Solid State Physics
IdentifiersURN: urn:nbn:se:uu:diva-159175DOI: 10.1016/j.bios.2011.08.019ISI: 000296174700031OAI: oai:DiVA.org:uu-159175DiVA: diva2:443095