Skin barrier disruption by sodium lauryl sulfate-exposure alters the expressions of involucrin, transglutaminase 1, profilaggrin, and kallikreins during the repair phase in human skin in vivo
2008 (English)In: Journal of Investigative Dermatology, ISSN 0022-202X, E-ISSN 1523-1747, Vol. 128, no 5, 1212-1219 p.Article in journal (Refereed) Published
Detergents are skin irritants affecting keratinocytes. In this study, healthy volunteers were exposed to water (vehicle) and 1% sodium lauryl sulfate (SLS) under occlusive patch tests for 24 hours. The messenger RNA (mRNA) expression of keratinocyte differentiation markers and of enzymes involved in corneodesmosome degradation was examined in skin biopsies (n=8) during the repair phase (6 hours to 7 days postexposure) using real-time reverse-transcription PCR. It was found that the expression of involucrin was increased at 6 hours, but then rapidly normalized. The expression of transglutaminase 1 exhibited a twofold increase after 24 hours in the SLS-exposed skin. Profilaggrin was decreased after 6 hours. Later (4-7 days), the expression in SLS-exposed areas was >50% above than in control areas. An increased and altered immunofluorescence pattern of involucrin, transglutaminase 1, and filaggrin was also found (n=4). At 6 hours post-SLS exposure, the mRNA expression of kallikrein-7 (KLK-7) and kallikrein-5 (KLK-5) was decreased by 50 and 75%, respectively, as compared with control and water-exposed areas. Thereafter, the expression pattern of KLK-7 and KLK-5 was normalized. Changes in protein expression of KLK-5 were also found. In conclusion, SLS-induced skin barrier defects induce altered mRNA expression of keratinocyte differentiation markers and enzymes degrading corneodesmosomes.
Place, publisher, year, edition, pages
2008. Vol. 128, no 5, 1212-1219 p.
Adult, Biopsy, Cell Differentiation/drug effects, Dermatitis; Irritant/pathology/*physiopathology, Female, Humans, Intermediate Filament Proteins/*genetics/metabolism, Irritants/toxicity, Kallikreins/*genetics/metabolism, Keratinocytes/drug effects/metabolism/pathology, Male, Middle Aged, Patch Tests, Protein Precursors/*genetics/metabolism, RNA; Messenger/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Skin/metabolism/pathology/physiopathology, Sodium Dodecyl Sulfate/toxicity, Transglutaminases/*genetics/metabolism, Wound Healing/physiology
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-16545DOI: 10.1038/sj.jid.5701170ISI: 000255250400021PubMedID: 18007579OAI: oai:DiVA.org:uu-16545DiVA: diva2:44316