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Determination of tafenoquine in dried blood spots and plasma using LC and fluorescence detection
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Center for Clinical Research Dalarna.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
2011 (English)In: Bioanalysis, ISSN 1757-6180, E-ISSN 1757-6199, Vol. 3, no 16, 1847-1853 p.Article in journal (Refereed) Published
Abstract [en]

Background: The growing problem of parasites developing resistance to the traditional antimalarial drugs makes the development of new effective and safe drugs crucial. Tafenoquine is a new promising antimalarial drug for prophylaxis and treatment.

Results: A bioanalytical method for the determination of tafenoquine in 100 mu l of capillary blood applied onto sampling paper and in 100 mu l of plasma has been developed and validated. The Whatman 31 ET Chr paper was treated with 0.6 mol/l tartaric acid to improve the extraction recovery and solid-phase extraction was used for cleanup procedure of the blood samples. Plasma samples were precipitated with methanol. Tafenoquine and internal standard were separated on a Zorbax SB-CN column by reversed-phase LC and detected with fluorescence detection at 262 and 470 nm. The within- and between-day variations were below 10 and 14%, respectively, over the range 50-200 nmol/l for capillary blood on sampling paper and below 6 and 10% for plasma samples. The LLOQ of the method was 50 nmol/l.

Conclusion: The developed method has adequate sensitivity and is highly suitable for clinical studies in dried blood spots and plasma.

Place, publisher, year, edition, pages
2011. Vol. 3, no 16, 1847-1853 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-159260DOI: 10.4155/BIO.11.173ISI: 000294653600014OAI: oai:DiVA.org:uu-159260DiVA: diva2:443480
Available from: 2011-09-26 Created: 2011-09-26 Last updated: 2017-12-08Bibliographically approved

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