Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth disease virus
2008 (English)In: Veterinary Microbiology, ISSN 0378-1135, E-ISSN 1873-2542, Vol. 127, no 3-4, 227-236 p.Article in journal (Refereed) Published
A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3 FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.
Place, publisher, year, edition, pages
2008. Vol. 127, no 3-4, 227-236 p.
Proximity ligation assay (PLA), Foot-and-mouth disease (FMD), Diagnostic test, Pathogen detection
IdentifiersURN: urn:nbn:se:uu:diva-16649DOI: 10.1016/j.vetmic.2007.08.026ISI: 000253831400002PubMedID: 17897794OAI: oai:DiVA.org:uu-16649DiVA: diva2:44420