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Mesenchymal stem cells stimulate antibody secretion in human B cells.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology.
2007 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 65, no 4, 336-343 p.Article in journal (Refereed) Published
Abstract [en]

Mesenchymal stem cells (MSC) have immunomodulatory effects and inhibit T-cell responses to alloantigens and mitogens in vitro and in vivo. We wanted to examine the effect of MSC on human B cells. MSC stimulated IgG production, measured in an enzyme-linked immunospot (ELIspot) assay in blood and spleen lymphocytes. MSC only induced a low proliferation. When a semipermeable membrane separated MSC and mononuclear cells, the IgG production was stimulated in unfractionated lymphocytes. In contrast, enriched B cells required cell contact with MSC to produce IgG. Co-cultures of MSC and lymphocytes increased IFN-γ production. MSC produce IL-6, and addition of MSC to spleen cells dramatically increased IL-6 levels. After lymphocyte stimulation with lipopolysaccharide (LPS), cytomegalovirus or varicella zoster virus, MSC either stimulated or inhibited IgG response, depending on the level of stimulation by LPS or the viral antigens. Similar results were obtained for enriched B cells. To conclude, MSC stimulate B-cell antibody secretion. The IgG secretion by activated B cells may be stimulated or inhibited by the addition of MSC, depending on the level of stimulation.

Place, publisher, year, edition, pages
2007. Vol. 65, no 4, 336-343 p.
Keyword [en]
Antibody Formation, B-Lymphocytes/*cytology/*immunology, Cell Communication/immunology, Coculture Techniques, Humans, Immunoglobulin G/*biosynthesis, Interferon Type II/biosynthesis, Interleukin-6/biosynthesis, Lymphocyte Activation/*immunology, Mesenchymal Stem Cells/*immunology
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-16684DOI: 10.1111/j.1365-3083.2007.01905.xPubMedID: 17386024OAI: oai:DiVA.org:uu-16684DiVA: diva2:44455
Available from: 2008-06-02 Created: 2008-06-02 Last updated: 2017-12-08Bibliographically approved

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Rasmusson, Ida

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