Encapsulation and diffraction-pattern-correction methods to reduce the effect of damage in x-ray diffraction imaging of single biological molecules
2007 (English)In: Physical Review Letters, ISSN 0031-9007, Vol. 98, no 19, 198302- p.Article in journal (Refereed) Published
Short and intense x-ray pulses may be used for atomic-resolution diffraction imaging of single biological molecules. Radiation damage and a low signal-to-noise ratio impose stringent pulse requirements. In this Letter, we describe methods for decreasing the damage and improving the signal by encapsulating the molecule in a sacrificial layer (tamper) that reduces atomic motion and by postprocessing the pulse-averaged diffraction pattern to correct for ionization damage. Simulations show that these methods greatly improve the image quality.
Place, publisher, year, edition, pages
2007. Vol. 98, no 19, 198302- p.
IdentifiersURN: urn:nbn:se:uu:diva-16728DOI: 10.1103/PhysRevLett.98.198302ISI: 000246413200062PubMedID: 17677667OAI: oai:DiVA.org:uu-16728DiVA: diva2:44499