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Encapsulation and diffraction-pattern-correction methods to reduce the effect of damage in x-ray diffraction imaging of single biological molecules
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
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2007 (English)In: Physical Review Letters, ISSN 0031-9007, Vol. 98, no 19, 198302- p.Article in journal (Refereed) Published
Abstract [en]

Short and intense x-ray pulses may be used for atomic-resolution diffraction imaging of single biological molecules. Radiation damage and a low signal-to-noise ratio impose stringent pulse requirements. In this Letter, we describe methods for decreasing the damage and improving the signal by encapsulating the molecule in a sacrificial layer (tamper) that reduces atomic motion and by postprocessing the pulse-averaged diffraction pattern to correct for ionization damage. Simulations show that these methods greatly improve the image quality.

Place, publisher, year, edition, pages
2007. Vol. 98, no 19, 198302- p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-16728DOI: 10.1103/PhysRevLett.98.198302ISI: 000246413200062PubMedID: 17677667OAI: oai:DiVA.org:uu-16728DiVA: diva2:44499
Available from: 2008-06-03 Created: 2008-06-03 Last updated: 2011-02-04Bibliographically approved

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Szöke, AbrahamTimneanu, Nicusor
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Molecular biophysicsDepartment of Cell and Molecular Biology
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