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Impact of an error in the column hold-up time for correct adsorption isotherm determination in chromatography: II. Can a wrong column porosity lead to a correct prediction of overloaded elution profiles?
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
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2008 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1194, no 2, 205-212 p.Article in journal (Refereed) Published
Abstract [en]

The adsorption isotherm was determined for phenol in methanol/water on a C-8 stationary phase using frontal analysis in staircase mode, assuming different total column porosities, from 1 to 87%. Each set of adsorption isotherm data, with a certain column porosity, was fitted to various adsorption models and the generated parameters were used to calculate overloaded elution band profiles that were compared with experiments. It was found that the bi-Langmuir model had an optimum fit for a porosity that corresponds well with the value found experimentally. The adsorption energy distribution (AED) calculations and error analysis confirmed a bimodal energy distribution. It was also found that band profiles can be accurately predicted with a quite arbitrary chosen porosity, under prerequisite that a wrong but flexible adsorption model is chosen instead of the correct one. The latter result is very useful for quick optimizations of preparative separations where the exact value of the column porosity is not available.

Place, publisher, year, edition, pages
2008. Vol. 1194, no 2, 205-212 p.
Keyword [en]
Column porosity, Hold-up volume, Void volume, Frontal analysis, Equilibrium isotherm, Ideal model, Adsorption energy distribution, Prediction of band profiles
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-16736DOI: 10.1016/j.chroma.2008.04.053ISI: 000257083600011PubMedID: 18499114OAI: oai:DiVA.org:uu-16736DiVA: diva2:44507
Available from: 2008-06-03 Created: 2008-06-03 Last updated: 2017-12-08Bibliographically approved

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Samuelsson, JörgenFornstedt, Torgny

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