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The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor beta-subunit
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Molecular Cell Biology.
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2011 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 412, no 3, 435-440 p.Article in journal (Refereed) Published
Abstract [en]

Inhibitory glycine receptors (GlyRs) are densely packed in the postsynaptic membrane due to a high-affinity interaction of their beta-subunits with the scaffolding protein gephyrin. Here, we used an affinity-based proteomic approach to identify the trafficking proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea) as novel GlyR beta-subunit (GlyR beta) interacting proteins in rat brain. Recombinant Vps35 and a central fragment of Nbea bound to the large intracellular loop of GlyR beta in glutathione-S-transferase pull-downs; in addition, Vps35 displayed binding to gephyrin. Immunocytochemical staining of spinal cord sections revealed Nbea immunoreactivity apposed to and colocalizing with marker proteins of inhibitory synapses. Our data are consistent with roles of Vps35 and Nbea in the retrieval and post-Golgi trafficking of synaptic GlyRs and possibly other neurotransmitter receptors.

Place, publisher, year, edition, pages
2011. Vol. 412, no 3, 435-440 p.
Keyword [en]
Glycine receptor, Spinal cord, Gephyrin, Neurobeachin, Retromer complex, Vacuolar Protein Sorting 35
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-159474DOI: 10.1016/j.bbrc.2011.07.110ISI: 000294835200007OAI: oai:DiVA.org:uu-159474DiVA: diva2:445485
Available from: 2011-10-04 Created: 2011-10-03 Last updated: 2017-12-08Bibliographically approved

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Kilimann, Manfred W.

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