uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Identification of a core set of 58 gene transcripts with broad and specific expression in the microvasculature.
Karolinska Institutet, Institutionen för medicinsk biokemi och biofysik.
Karolinska Institutet, Institutionen för medicinsk biokemi och biofysik.
Show others and affiliations
2008 (English)In: Arteriosclerosis, Thrombosis and Vascular Biology, ISSN 1079-5642, E-ISSN 1524-4636, Vol. 28, no 8, 1469-76 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE: Pathological angiogenesis is an integral component of many diseases. Antiangiogenesis and vascular targeting are therefore promising new therapeutic principles. However, few endothelial-specific putative drug targets have been identified, and information is still limited about endothelial-specific molecular processes. Here we aimed at determining the endothelial cell-specific core transcriptome in vivo.

METHODS AND RESULTS: Analysis of publicly available microarray data identified a mixed vascular/lung cluster of 132 genes that correlated with known endothelial markers. Filtering against kidney glomerular/nonglomerular and brain vascular/nonvascular microarray profiles separated contaminating lung markers, leaving 58 genes with broad and specific microvascular expression. More than half of these have not previously been linked to endothelial functions or studied in detail before. The endothelial cell-specific expression of a selected subset of these, Eltd1, Gpr116, Ramp2, Slc9a3r2, Slc43a3, Rasip1, and NM_023516, was confirmed by real-time quantitative polymerase chain reaction and/or immunohistochemistry.

CONCLUSIONS: We have used a combination of publicly available and own microarray data to identify 58 gene transcripts with broad yet specific expression in microvascular endothelium. Most of these have unknown functions, but many of them are predicted to be cell surface expressed or implicated in cell signaling processes and should therefore be explored as putative microvascular drug targets.

Place, publisher, year, edition, pages
2008. Vol. 28, no 8, 1469-76 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-159703DOI: 10.1161/ATVBAHA.108.165738PubMedID: 18483404OAI: oai:DiVA.org:uu-159703DiVA: diva2:446362
Available from: 2011-10-07 Created: 2011-10-07 Last updated: 2011-10-07

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hellström, Mats
In the same journal
Arteriosclerosis, Thrombosis and Vascular Biology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 256 hits
ReferencesLink to record
Permanent link

Direct link