uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells
Show others and affiliations
2011 (English)In: Journal of Biomedical Science, ISSN 1021-7770, E-ISSN 1423-0127, Vol. 18, 59- p.Article in journal (Refereed) Published
Abstract [en]

Background: Developing efficient methods to isolate and identify human adipose-derived mesenchymal stem cells (hADSCs) remains to be one of the major challenges in tissue engineering.

Methods: We demonstrate here a method by isolating hADSCs from abdominal subcutaneous adipose tissue harvested during caesarian section. The hADSCs were isolated from human adipose tissue by collagenase digestion and adherence to flasks.

Results: The yield reached around 1 x 10(6) hADSCs per gram adipose tissue. The following comprehensive identification and characterization illustrated pronounced features of mesenchymal stem cells (MSCs). The fibroblast-like hADSCs exhibited typical ultrastructure details for vigorous cell activities. Karyotype mapping showed normal human chromosome. With unique immunophenotypes they were positive for CD29, CD44, CD73, CD105 and CD166, but negative for CD31, CD34, CD45 and HLA-DR. The growth curve and cell cycle analysis revealed high capability for self-renewal and proliferation. Moreover, these cells could be functionally induced into adipocytes, osteoblasts, and endothelial cells in the presence of appropriate conditioned media.

Conclusion: The data presented here suggest that we have developed high efficient isolation and cultivation methods with a systematic strategy for identification and characterization of hADSCs. These techniques will be able to provide safe and stable seeding cells for research and clinical application.

Place, publisher, year, edition, pages
2011. Vol. 18, 59- p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-159797DOI: 10.1186/1423-0127-18-59ISI: 000295049200001OAI: oai:DiVA.org:uu-159797DiVA: diva2:447008
Available from: 2011-10-10 Created: 2011-10-10 Last updated: 2011-10-10Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Department of Medical Cell Biology
In the same journal
Journal of Biomedical Science
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 184 hits
ReferencesLink to record
Permanent link

Direct link