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Mesoporous titanium dioxide coating for metallic implants
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
BIOMATCELL, VINN excellence Center of Biomaterials and Cell Therapy, Göteborg, Dept of Biomaterials, Institute for Clinical Sciences, Sahlgrenska Academy at University of Gothenburg, Göteborg.
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2012 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 100B, no 1, 82-93 p.Article in journal (Refereed) Published
Abstract [en]

A bioactive mesoporous titanium dioxide (MT) coating for surface drug delivery has been investigated to develop a multifunctional implant coating, offering quick bone bonding and biological stability. An evaporation induced self-assembly (EISA) method was used to prepare a mesoporous titanium dioxide coating of the anatase phase with BET surface area of 172 m2/g and average pore diameter of 4.3 nm. Adhesion tests using the scratch method and an in situ screw-in/screw-out technique confirm that the MT coating bonds tightly with the metallic substrate, even after removal from bone. Because of its high surface area, the bioactivity of the MT coating is much better than that of a dense TiO2 coating of the same composition. Quick formation of hydroxyapatite (HA) in vitro can be related to enhance bonding with bone. The uptake of antibiotics by the MT coating reached 13.4 mg/cm3 within a 24 h loading process. A sustained release behavior has been obtained with a weak initial burst. By using Cephalothin as a model drug, drug loaded MT coating exhibits a sufficient antibacterial effect on the material surface, and within millimeters from material surface, against E.coli. Additionally, the coated and drug loaded surfaces showed no cytotoxic effect on cell cultures of the osteoblastic cell line MG-63. In conclusion, this study describes a novel, biocompatiblemesoporous implant coating, which has the ability to induce HA formation and could be used as a surface drug-delivery system.

Place, publisher, year, edition, pages
2012. Vol. 100B, no 1, 82-93 p.
Keyword [en]
mesoporous materials, titanium oxide, drug delivery, bioactivity, implant coating
National Category
Biomaterials Science Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Materials Science
Identifiers
URN: urn:nbn:se:uu:diva-159881DOI: 10.1002/jbm.b.31925ISI: 000297949800010OAI: oai:DiVA.org:uu-159881DiVA: diva2:447302
Available from: 2011-10-11 Created: 2011-10-11 Last updated: 2017-12-08Bibliographically approved
In thesis
1. Nanoscale Osseointegration: Characterization of Biomaterials and their Interfaces with Electron Tomography
Open this publication in new window or tab >>Nanoscale Osseointegration: Characterization of Biomaterials and their Interfaces with Electron Tomography
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bone response is one of the key determining factors in the overall success of biomaterials intended for bone regeneration and osseointegration. Understanding the formation of bone at an implant surface may lead to the improved design of biomaterials for the future. However, due to the inhomogeneity of bone tissue at an interface, two-dimensional images often lack detail on the interfacial complexity. Furthermore, the increasing use of nanotechnology in the design and production of biomaterials demands characterization techniques on a similar nano length scale.

While current analysis methods, such as X-ray tomography, transmission electron microscopy, focused ion beam microscopy and scanning electron microscopy, provide a basis for analysing biomaterials and biointerfaces, they are incapable of doing so with both nanometre resolution and three-dimensional clarity. In contrast, electron tomography may be used to characterize the three-dimensional structure of biomaterials and their interfaces to bone with nanometre resolution.

In this work, hydroxyapatite scaffolds, and laser-modified titanium and Ti6Al4V implants were studied in contact with human or rabbit bone. Z-contrast electron tomography revealed that the orientation of collagen in bone apposing hydroxyapatite, titanium and Ti6Al4V implants is consistently parallel to the implant surface, where the bioactive layer that precipitates on HA is oriented perpendicular to the implant surface. With this method, complete three-dimensional nanoscale osseointegration of titanium-based implants was also established.

The extension of this technique from interfacial analyses to the design of biomaterials provided an understanding of the pore structure of mesoporous titania. In further investigations, the open three-dimensional pore network, as revealed by electron tomography, showed promise as a coating that improves implant osseointegration and enables site-specific drug-delivery from an implant surface.

In summary, it was demonstrated that two-dimensional characterization techniques were insufficient for the investigation of nanostructured biomaterials, as well as their interfaces to bone. Visualizing biointerfaces and biomaterials with nanometre precision in three-dimensions can expose new fundamental information on materials properties and bone response, enabling better design of biomaterials for the future.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2012. 61 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 962
Keyword
Electron tomography, transmission electron microscopy, hydroxyapatite, titanium, titania, bone, implant, osseointegration, interface, mesoporous
National Category
Materials Engineering
Research subject
Materials Science
Identifiers
urn:nbn:se:uu:diva-179445 (URN)978-91-554-8441-5 (ISBN)
Public defence
2012-10-05, Polhemsalen, Ångström Laboratory, Lägerhyddsvägen 1, Uppsala, 10:15 (English)
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Available from: 2012-09-14 Created: 2012-08-15 Last updated: 2013-01-22Bibliographically approved

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Xia, WeiGrandfield, KathrynHoess, AndreasCai, YanlingEngqvist, Håkan

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