U-2973, a novel B-cell line established from a patient with a mature B-cell leukemia displaying concurrent t(14;18) and MYC translocation to a non-IG gene partner
2008 (English)In: European Journal of Haematology, ISSN 0902-4441, E-ISSN 1600-0609, Vol. 81, no 3, 218-225 p.Article in journal (Refereed) Published
B-cell lymphomas/leukemias with simultaneous t(14;18)(q32;q21) and MYC rearrangements have recently been shown to constitute a separate diagnostic entity, presenting with a rapid clinical course and a very poor prognosis. We describe the establishment of an Epstein-Barr virus negative cell line, designated U-2973, from a male patient with a de novo aggressive B-cell lymphoma/leukemia and very high peripheral blast cell count. Flow cytometry of bone marrow cells and U-2973 displayed a mature B-cell phenotype, and immunostaining showed expression of MYC and BCL2. IG gene rearrangement data were consistent with a lymphoid neoplasm of germinal centre derivation. Cytogenetic studies using conventional G-banding, fluorescent in situ hybridization, spectral karyotyping and single nucleotide polymorphism array demonstrated a complex karyotype with both a t(14;18) and double translocations between MYC and a non-IG gene partner located at chromosome 12p12.1.
Place, publisher, year, edition, pages
2008. Vol. 81, no 3, 218-225 p.
t(14;18), IGH/BCL2, MYC, B-cell, lymphoma, leukemia, cell line
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-16962DOI: 10.1111/j.1600-0609.2008.01098.xISI: 000258325600007PubMedID: 18510704OAI: oai:DiVA.org:uu-16962DiVA: diva2:44733