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Na(+)-stimulated nitrate uptake with increased activity under osmotic upshift in Synechocystis sp strain PCC 6803
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Molecular Biomimetics.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Photochemistry and Molecular Science, Molecular Biomimetics.
2011 (English)In: World Journal of Microbiology & Biotechnology, ISSN 0959-3993, E-ISSN 1573-0972, Vol. 27, no 10, 2467-2473 p.Article in journal (Refereed) Published
Abstract [en]

In the non-diazotrophic cyanobacterium Synechocystis sp. strain PCC 6803, an osmolality of 30 and 40 mosmol/kg sorbitol and NaCl resulted in 3.5- and 4.5-fold increase of nitrate uptake, respectively. The NaCl-stimulated uptake was abolished by treatment with chloramphenicol. At 25 mosmol/kg or higher, NaCl induced higher nitrate uptake than sorbitol suggesting an ionic effect of Na(+). The nitrate uptake in Synechocystis showed K (s) and V (max) values of 46 mu M and 1.37 mu mol/min/mg Chl, respectively. Mutants disrupted in nitrate and nitrite reductase exhibited a decreased nitrate uptake. Ammonium, chlorate, and dl-glyceraldehyde caused a reduction of nitrate uptake. Dark treatment caused a drastic reduction of uptake by 70% suggesting an energy-dependent system. Nitrate transport was sensitive to various metabolic inhibitors including those dissipating proton gradients and membrane potential. The results suggest that nitrate uptake in Synechocystis is stimulated by Na(+) ions and requires energy provided by the functioning electron transport chain.

Place, publisher, year, edition, pages
2011. Vol. 27, no 10, 2467-2473 p.
Keyword [en]
Cyanobacteria, Nitrate uptake, Osmotic upshift, Synechocystis
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-159780DOI: 10.1007/s11274-011-0706-6ISI: 000294966900028OAI: oai:DiVA.org:uu-159780DiVA: diva2:447726
Available from: 2011-10-13 Created: 2011-10-10 Last updated: 2017-12-08Bibliographically approved

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Lindblad, Peter

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