Optimering och validering av realtids-PCR assay för detektion av verocytotoxin-producerande E. coli (VTEC)
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Pathogenic E. coli such as VTEC can cause serious diseases to humans. A detection method for VTEC based on realtime-PCR has been developed by EU-RL VTEC (European Union Reference Laboratory VTEC) and the National Food Administration. In this master thesis I have optimized and validated a part of the method which detects serotypes of VTEC. During optimization the primer concentration, probe concentration and annealing temperature were evaluated for a more sensitive detection. Validation showed that the PCR-reaction is both effective and sensitive (LOD100). Validation of the whole method did not give any significant results due to the fact the sensitivity (LOD50) could not be determined.
Place, publisher, year, edition, pages
2011. , 33 p.
UPTEC X, 11035
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-160192OAI: oai:DiVA.org:uu-160192DiVA: diva2:448689
Molecular Biotechnology Engineering Programme
Josefsson, Lars-Göran, Associate Professor in Molecular Cell Biology
Krabbe, Margareta, Universitetslektor