Normal levels of constitutive and death receptor-mediated apoptosis of peripheral blood neutrophils from patients with chronic idiopathic neutropenia.
2007 (English)In: Clinical Immunology, ISSN 1521-6616, Vol. 122, no 3, 349-355 p.Article in journal (Refereed) Published
To investigate the role of neutrophil apoptosis in the pathogenesis of chronic neutropenia, we examined constitutive and death receptor-mediated apoptosis ex vivo of peripheral blood neutrophils obtained from six chronic idiopathic neutropenia (CIN) patients and six healthy adult blood donors. Apoptosis was quantified based on phosphatidylserine externalization and caspase-3 activation in freshly isolated neutrophils or after overnight cultivation of neutrophils in the absence or presence of pro- or anti-apoptotic factors, including the pan-caspase inhibitor, zVAD-fmk. Neutrophils from CIN patients receiving treatment with granulocyte colony-stimulating factor appeared to be more prone to constitutive apoptosis than cells from untreated patients; however, further investigations in larger cohorts of patients are needed to validate these pilot studies. Overall, the level of neutrophil apoptosis was similar in patient and control groups, thus supporting the notion that the underlying defect in these neutropenia patients lies elsewhere, such as in the bone marrow microenvironment.
Place, publisher, year, edition, pages
2007. Vol. 122, no 3, 349-355 p.
Adult, Aged; 80 and over, Apoptosis/*immunology, Cells; Cultured, Chronic Disease, Female, Humans, Male, Middle Aged, Neutropenia/immunology/metabolism/*pathology, Neutrophils/immunology/*metabolism/*pathology, Receptors; Death Domain/blood/*physiology
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-17299DOI: 10.1016/j.clim.2006.10.015PubMedID: 17188576OAI: oai:DiVA.org:uu-17299DiVA: diva2:45070
Publikation från annat universitet2008-06-182008-06-182015-05-08Bibliographically approved