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Analysis of VEGF-A Regulated Gene Expression in Endothelial Cells to Identify Genes Linked to Angiogenesis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
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2011 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 6, no 9, e24887- p.Article in journal (Refereed) Published
Abstract [en]

Angiogenesis is important for many physiological processes, diseases, and also regenerative medicine. Therapies that inhibit the vascular endothelial growth factor (VEGF) pathway have been used in the clinic for cancer and macular degeneration. In cancer applications, these treatments suffer from a "tumor escape phenomenon'' where alternative pathways are upregulated and angiogenesis continues. The redundancy of angiogenesis regulation indicates the need for additional studies and new drug targets. We aimed to (i) identify novel and missing angiogenesis annotations and (ii) verify their significance to angiogenesis. To achieve these goals, we integrated the human interactome with known angiogenesis-annotated proteins to identify a set of 202 angiogenesis-associated proteins. Across endothelial cell lines, we found that a significant fraction of these proteins had highly perturbed gene expression during angiogenesis. After treatment with VEGF-A, we found increasing expression of HIF-1 alpha, APP, HIV-1 tat interactive protein 2, and MEF2C, while endoglin, liprin beta 1 and HIF-2 alpha had decreasing expression across three endothelial cell lines. The analysis showed differential regulation of HIF-1 alpha and HIF-2 alpha. The data also provided additional evidence for the role of endothelial cells in Alzheimer's disease.

Place, publisher, year, edition, pages
2011. Vol. 6, no 9, e24887- p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-160416DOI: 10.1371/journal.pone.0024887ISI: 000295321800074OAI: oai:DiVA.org:uu-160416DiVA: diva2:450886
Available from: 2011-10-24 Created: 2011-10-24 Last updated: 2017-12-08Bibliographically approved

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