uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Lys Tag: an easy and robust chemical modification for improved de novo sequencing with a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
2008 (English)In: Rapid Communications in Mass Spectrometry, ISSN 0951-4198, Vol. 22, no 12, 1823-33 p.Article in journal (Refereed) Published
Abstract [en]

Mass spectrometry using a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) instrument is a widespread technique for various types of proteomic analysis. Along with an expanding interest in proteomics, there is a strong requirement for the identification of proteins with high confidence from biological samples. Peptide modification by a wide variety of post-translational modifications (PTMs), the existence of different protein isoforms and the presence of uncharacterized genomes of many species, make protein identification through peptide mass fingerprinting (PMF) often unachievable. Peptide de novo sequencing has been proven to be a useful approach to overcome these variables, and efficient derivatization processes are important tools to achieve this goal. In the present work we describe the methodology and experimental applications of a fast, efficient and cheap lysine derivatization. This chemical modification improves the signals from lysine-terminated peptides and can be efficiently used as a lysine-blocking agent in combination with other derivatization techniques. Most importantly, upon peptide fragmentation it generates a neat series of predominantly y-ions, allowing the determination of unambiguous amino acid sequences. Moreover, this chemical compound was used with target-eluted samples, enabling a second, alternative analysis of the same sample in the MALDI mass spectrometer.

Place, publisher, year, edition, pages
2008. Vol. 22, no 12, 1823-33 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-17572DOI: 10.1002/rcm.3555ISI: 000256985100008PubMedID: 18470875OAI: oai:DiVA.org:uu-17572DiVA: diva2:45343
Available from: 2008-07-10 Created: 2008-07-10 Last updated: 2009-10-30Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Hellman, Ulf
By organisation
Ludwig Institute for Cancer Research
In the same journal
Rapid Communications in Mass Spectrometry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 178 hits
ReferencesLink to record
Permanent link

Direct link