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HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 17177 Stockholm, Sweden.
Institute for Medical Biometry and Statistics, University of Lübeck, 23538 Lübeck, Germany.
Institute for Pathology, University Clinic RWTH Aachen, 52074 Aachen, Germany.
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2011 (English)In: Cellular and Molecular Life Sciences (CMLS), ISSN 1420-682X, E-ISSN 1420-9071, Vol. 68, no 19, 3261-3274 p.Article in journal (Refereed) Published
Abstract [en]

DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies. Further understanding of the translation of DNA aneuploidy into protein expression will help to define novel biomarkers to improve therapies and prognosis. DNA ploidy was assessed by image cytometry. Comparison of gel-electrophoresis-based protein expression patterns of three diploid and four aneuploid colorectal cancer cell lines detected 64 ploidy-associated proteins. Proteins were identified by mass spectrometry and subjected to Ingenuity Pathway Analysis resulting in two overlapping high-ranked networks maintaining Cellular Assembly and Organization, Cell Cycle, and Cellular Growth and Proliferation. CAPZA1, TXNL1, and HDAC2 were significantly validated by Western blotting in cell lines and the latter two showed expression differences also in clinical samples using a tissue microarray of normal mucosa (n=19), diploid (n=31), and aneuploid (n=47) carcinomas. The results suggest that distinct protein expression patterns, affecting TXNL1 and HDAC2, distinguish aneuploid with poor prognosis from diploid colorectal cancers.

Place, publisher, year, edition, pages
Springer , 2011. Vol. 68, no 19, 3261-3274 p.
Keyword [en]
Colon cancer, Genomic instability, Two-dimensional gel electrophoresis, Mass spectrometry, Aneuploidy, HDAC2
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-161244DOI: 10.1007/s00018-011-0628-3PubMedID: 21290163OAI: oai:DiVA.org:uu-161244DiVA: diva2:455499
Available from: 2011-11-10 Created: 2011-11-10 Last updated: 2017-12-08Bibliographically approved

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