Vitrification with DMSO protects embryo membrane integrity better than solutions without DMSO
2008 (English)In: Reproductive Biomedicine Online, ISSN 1472-6483, Vol. 17, no 3, 378-384 p.Article in journal (Refereed) Published
Vitrification has become common for cryopreservation of embryos. However, the most Optimal protocol for Vitrification is still to be found. Two vitrification protocols with similar osmolarities were compared: Protocol A. containing dimethyl sulphoxide (DMSO), propane-2-diol, and ethylene glycol. and Protocol B. containing propane-2-diol and ethylene glycol. Viability and the importance of specific incubation times for early embryo recovery. survival. and cleavage were studied. For assessment of cryodamage, embryos were labelled with Alexa Fluor 488-conjugated annexin V and propidium iodide. Vitrification Studies Oil early mouse embryos were followed Up with studies oil human embryos,. The two vitrification protocols did not differ ill embryo survival rates and were equally efficient ill both mouse and human embryo models. Morphological assessment of embryos directly after vitrification was not a useful tool for assessing survival in this study. Extended exposure of embryos with both vitrification protocols showed that the DMSO-containing vitrification solutions did not lead to cell membrane damage and death as quickly as the DMSO-free vitrification solutions. To assess embryo viability, the authors recommend that vitrification of early embryos should be combined with extended culture and assessment of normal blastocyst development before transferring to patients.
Place, publisher, year, edition, pages
2008. Vol. 17, no 3, 378-384 p.
Cryodamage, cryoloop, fluorescence imaging, post-vitrification survival, vitrification
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-17797ISI: 000259031400011PubMedID: 18765008OAI: oai:DiVA.org:uu-17797DiVA: diva2:45568