Protein A and protein G ELISA for the detection of IgG autoantibodies against tissue transglutaminase in childhood celiac disease
2008 (English)In: Clinica Chimica Acta, ISSN 0009-8981, Vol. 395, no 1-2, 72-6 p.Article in journal (Refereed) Published
Objectives: To investigate if the detection of celiac disease (CD) in children was improved by using alternative conjugates for assessment of tissue transglutaminase (tTG) autoantibodies. Methods: Serum samples from 108 biopsy confirmed CD children and 42 control subjects were investigated for the presence of autoantibodies with tTG coated microplates using protein A (PA), protein G (PG), anti-IgG, or anti-IgA as conjugates. Results: Of the 108 CD children, 86 (80%) were IgG-tTG positive, 91 (84%) were positive with the PA-conjugate, 94 (87%) were positive with the PG-conjugate, and 103 (95%) were IgA-tTG positive. Among the 42 controls. 4 (10%) were IgG-tTG positive, 5 (12%) were positive with both the PA- and PG conjugates. whereas 3 (7%) were IgA-tTG positive. Compared with IgG-tTG the concordance was 93% for PA and 95% for PG, with a positive correlation between antibody levels (r=0.967 and r=0.975. p< ;0.0001). All but one CD child were found positive by combining IgG-tTG and IgA-tTG detection. Conclusions: The sensitivity of IgG-tTG detection with ELISA increased by protein A or protein G conjugates, whereas the specificity was reduced as compared with anti-IgG conjugate. The combined measurement of IgA-tTG and IgG-tTG still seems to be the optimal procedure when screening children for CD.
Place, publisher, year, edition, pages
2008. Vol. 395, no 1-2, 72-6 p.
celiac disease, ELISA, protein A, protein G, tissue transglutaminase
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-17807DOI: 10.1016/j.cca.2008.05.005ISI: 000258799300014PubMedID: 18514068OAI: oai:DiVA.org:uu-17807DiVA: diva2:45578