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Inactivation of Pmel Alters Melanosome Shape But Has Only a Subtle Effect on Visible Pigmentation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience, Developmental Neuroscience.
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2011 (English)In: PLoS Genetics, ISSN 1553-7390, Vol. 7, no 9, e1002285- p.Article in journal (Refereed) Published
Abstract [en]

PMEL is an amyloidogenic protein that appears to be exclusively expressed in pigment cells and forms intralumenal fibrils within early stage melanosomes upon which eumelanins deposit in later stages. PMEL is well conserved among vertebrates, and allelic variants in several species are associated with reduced levels of eumelanin in epidermal tissues. However, in most of these cases it is not clear whether the allelic variants reflect gain-of-function or loss-of-function, and no complete PMEL loss-of-function has been reported in a mammal. Here, we have created a mouse line in which the Pmel gene has been inactivated (Pmel(-/-)). These mice are fully viable, fertile, and display no obvious developmental defects. Melanosomes within Pmel(-/-) melanocytes are spherical in contrast to the oblong shape present in wild-type animals. This feature was documented in primary cultures of skin-derived melanocytes as well as in retinal pigment epithelium cells and in uveal melanocytes. Inactivation of Pmel has only a mild effect on the coat color phenotype in four different genetic backgrounds, with the clearest effect in mice also carrying the brown/Tyrp1 mutation. This phenotype, which is similar to that observed with the spontaneous silver mutation in mice, strongly suggests that other previously described alleles in vertebrates with more striking effects on pigmentation are dominant-negative mutations. Despite a mild effect on visible pigmentation, inactivation of Pmel led to a substantial reduction in eumelanin content in hair, which demonstrates that PMEL has a critical role for maintaining efficient epidermal pigmentation.

Place, publisher, year, edition, pages
2011. Vol. 7, no 9, e1002285- p.
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-161066DOI: 10.1371/journal.pgen.1002285ISI: 000295419100034OAI: oai:DiVA.org:uu-161066DiVA: diva2:456359
Available from: 2011-11-14 Created: 2011-11-07 Last updated: 2011-11-15Bibliographically approved

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Hellström, Anders R.Fard, Shahrzad ShiraziLarsson, JimmyKullander, KlasKerje, SusanneHallböök, FinnAndersson, Leif
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Department of Medical Biochemistry and MicrobiologyScience for Life Laboratory, SciLifeLabDevelopmental NeuroscienceCancer and Vascular BiologyDevelopmental Genetics
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