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Human Cellular Immune Response Against Giardia lamblia 5 Years After Acute Giardiasis
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
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2011 (English)In: Journal of Infectious Diseases, ISSN 0022-1899, Vol. 204, no 11, 1779-1786 p.Article in journal (Refereed) Published
Abstract [en]

Background. Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic area made it possible to evaluate the long-term cellular mediated immunity and its specificity toward the 2 Giardia assemblages known to infect humans.

Methods. Peripheral blood mononuclear cells from 19 individuals infected with Giardia assemblage B 5 years previously and from 10 uninfected controls were cultured with antigens from assemblage A and B Giardia trophozoites for 6 days. Cell-mediated immunity was measured by a (3)H-thymidine proliferation assay and flow cytometric analysis of activation markers HLA-DR, CD45RO, CD25, and CD26 in T-cell subsets.

Results. Proliferation responses were significantly elevated in the group previously exposed to Giardia for nearly all Giardia antigens tested. Individual responses toward Giardia trophozoite whole cell, cytosolic, and excretory-secretory antigens from both assemblages correlated well. Activation marker responses were mainly seen in CD4 T cells.

Conclusions. G. lamblia infection induces long-term, albeit variable, cellular immune responses that are not assemblage specific and that are largely driven by CD4 T-cell activation.

Place, publisher, year, edition, pages
2011. Vol. 204, no 11, 1779-1786 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-161424DOI: 10.1093/infdis/jir639ISI: 000296297900018OAI: oai:DiVA.org:uu-161424DiVA: diva2:457550
Available from: 2011-11-18 Created: 2011-11-14 Last updated: 2011-11-18Bibliographically approved

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