uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Different interactions of cardiac and skeletal muscle ryanodine receptors with FK-506 binding protein isoforms
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology. (Barg)
1997 (English)In: American Journal of Physiology, ISSN 0002-9513, Vol. 272, no 5 Pt 1, C1726-C1733 p.Article in journal (Refereed) Published
Abstract [en]

In the present study, we compare functional consequences of dissociation and reconstitution of binding proteins FKBP12 and FKBP12.6 with ryanodine receptors from cardiac (RyR2) and skeletal muscle (RyR1). The skeletal muscle RyR1 channel became activated on removal of endogenously bound FKBP12, consistent with previous reports. Both FKBP12 and FKBP12.6 rebind to FKBP-depleted RyR1 and restore its quiescent channel behavior by altering ligand sensitivity, as studied by single-channel recordings in planar lipid bilayers, and macroscopic behavior of the channels (ryanodine binding and net energized Ca2- uptake). By contrast, removal of FKBP12.6 from the cardiac RyR2 did not modulate the function of the channel using the same types of assays as for RyR1. FKBP12 or FKBP12.6 had no effect on channel activity of FKBP12.6-depleted cardiac RyR2, although FKBP12.6 rebinds. Our studies reveal important differences between the two ryanodine receptor isoforms with respect to their functional interaction with FKBP12 and FKBP12.6.

Place, publisher, year, edition, pages
1997. Vol. 272, no 5 Pt 1, C1726-C1733 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-161889PubMedID: 9176165OAI: oai:DiVA.org:uu-161889DiVA: diva2:457768
Available from: 2011-11-19 Created: 2011-11-19 Last updated: 2011-11-21Bibliographically approved

Open Access in DiVA

No full text

Other links


Search in DiVA

By author/editor
Barg, Sebastian
By organisation
Department of Medical Cell Biology
In the same journal
American Journal of Physiology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 212 hits
ReferencesLink to record
Permanent link

Direct link