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Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens
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2006 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 62, no 10, 1196-1207 p.Article in journal (Refereed) Published
Abstract [en]

The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (approximately 220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.

Place, publisher, year, edition, pages
2006. Vol. 62, no 10, 1196-1207 p.
Keyword [en]
Automation, Bacteria, Cloning, Expression, Miniaturization, Viruses
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-18240DOI: 10.1107/S0907444906030915ISI: 000240618000009PubMedID: 17001096OAI: oai:DiVA.org:uu-18240DiVA: diva2:46012
Available from: 2008-01-29 Created: 2008-01-29 Last updated: 2011-06-16Bibliographically approved

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