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Adipose tissue processed for lipoinjection shows increased cellular survival in vitro when tissue engineering principles are applied.
Kirurgiska vetenskaper, KI.ORCID iD: 0000-0002-9735-1434
Kirurgiska vetenskaper, KI.
2002 (English)In: Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, ISSN 2000-656X, E-ISSN 2000-6764, Vol. 36, no 3, 166-71 p.Article in journal (Refereed) Published
Abstract [en]

Correcting soft tissue defects by autologous fat grafting is a routine procedure in plastic surgery. Its efficacy and safety has been discussed extensively and several techniques of lipoinjection have been developed. However, one is bound to overcorrect by 30%-70% or need to repeat the procedure because of resorption of the transplant. The reasons are that many of the transplanted cells are already differentiated, and also that there is no nutritional support to the inner cell layers when they are transplanted as fragments. By culturing autologous adipocytes one can ensure that only non-differentiated, but committed, preadipocytes are transplanted and the procedure can be done in a way that ensures optimal nutritional support for the cells. In the present study we have compared our cell culture technique with two common clinical ways of processing liposuction material and found that (pre)adipocytes survive and proliferate significantly better in cell culture.

Place, publisher, year, edition, pages
2002. Vol. 36, no 3, 166-71 p.
National Category
Surgery Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-162784PubMedID: 12141205OAI: oai:DiVA.org:uu-162784DiVA: diva2:461967
Available from: 2011-12-06 Created: 2011-12-05 Last updated: 2017-12-08
In thesis
1. In vitro and in vivo studies of tissue engineering in reconstructive plastic surgery
Open this publication in new window or tab >>In vitro and in vivo studies of tissue engineering in reconstructive plastic surgery
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Linköping: Unitryck, 2005. 75 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 905
National Category
Surgery
Research subject
Plastic Surgery
Identifiers
urn:nbn:se:uu:diva-286421 (URN)91-85299-06-5 (ISBN)
Public defence
2005-09-02, Linköping, 14:43 (English)
Opponent
Supervisors
Available from: 2016-04-22 Created: 2016-04-20 Last updated: 2016-04-22Bibliographically approved

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Huss, Fredrik R M

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