Change of bone tissue composition and impaired bone strength in rats exposed to 3,3',4,4',5-pentachlorobiphenyl (PCB126)
2000 (English)In: Toxicology, ISSN 0300-483X, E-ISSN 1879-3185, Vol. 150, no 1-3, 41-51 p.Article in journal (Refereed) Published
In previous studies we have described structural and functional changes in rat bone tissue caused by 3,3′,4,4′,5-pentachlorobiphenyl (PCB126). Some of the effects caused by PCB126 resemble those found in vitamin C-deficient rats, as well as those found in rats with a high dietary intake of vitamin A. The present investigation was designed to determine if these PCB126-induced changes could be inhibited by addition of vitamin C to the drinking water and if they could be evoked by vitamin A administration. Five groups of female rats were used in this study, which lasted for 12 weeks. Three of the groups were exposed to PCB126 (total dose 320 μg/kg, bw), either alone or in combination with vitamin C added to the drinking water (1 and 10 g/l, respectively). One group was given feed with increased level of vitamin A (600 000 U/kg pellet) and the fifth group served as controls. Using peripheral quantitative computed tomography (pQCT), it was found that PCB126 increased trabecular density and cortical thickness, but reduced the trabecular area. Furthermore, maximum torque and stiffness of the humerus during torsional testing and serum osteocalcin levels were reduced by PCB126. Of the PCB126 induced effects observed, addition of vitamin C only inhibited the reduction of serum osteocalcin. Like PCB126 vitamin A supplementation increased the inorganic content and the bone density and also reduced the trabecular area and polar moment of inertia but did not increase the cortical thickness or reduce maximum torque, stiffness or serum osteocalcin level. Apparently, the effects induced by PCB126 are not mediated either via decreased vitamin C level or increased vitamin A level.
Place, publisher, year, edition, pages
2000. Vol. 150, no 1-3, 41-51 p.
Animals, Biomechanics, Bone and Bones/chemistry/*drug effects/metabolism, Collagen/biosynthesis, Environmental Pollutants/*toxicity, Female, Organ Size/drug effects, Ovariectomy, Polychlorinated Biphenyls/*toxicity, Rats, Rats; Sprague-Dawley, Vitamin A/analysis
IdentifiersURN: urn:nbn:se:uu:diva-18464PubMedID: 10996662OAI: oai:DiVA.org:uu-18464DiVA: diva2:46236