Human Primary Bronchial Epithelial Cells are more Responsive to Titanium Dioxide Nanoparticles than the Lung Epithelial Cell Lines A549 and BEAS-2B
2012 (English)In: Nanotoxicology, ISSN 1743-5390, E-ISSN 1743-5404, Vol. 6, no 6, 623-634 p.Article in journal (Refereed) Published
We have compared the cellular uptake and responses of fivepreparations of nanocrystalline titanium dioxide (TiO2) betweennormal human bronchial epithelial (NHBE) cells and epithelialcell lines (A549 and BEAS-2B). The P25 nanoparticles, containingboth anatase and rutile modifications, induced reactive oxygenspecies (ROS) and secretion of the neutrophil chemoattractantIL-8 in all three cell types used. Pure anatase and rutile particlesprovoked differential IL-8 response in A549 and no response inBEAS-2B cells despite similar formation of ROS. The pure TiO2modifications also provoked release of the inflammatorymediators: IL-6, G-CSF and VEGF, in NHBE cells but not in the twocell lines. We conclude that the responsiveness of lung epithelialcells is strongly dependent on both the physicochemicalproperties of TiO2 nanoparticles and the type of responder cells.The differential pro-inflammatory responsiveness of primarylung epithelial cells compared with immortalized cell linesshould be considered in the assessment of adverse reactions toinhaled nanoparticles.
Place, publisher, year, edition, pages
Informa Healthcare, 2012. Vol. 6, no 6, 623-634 p.
TiO2 nanoparticles, normal human bronchial
Other Health Sciences Engineering and Technology
Research subject Toxicology; Engineering Science with specialization in Solid State Physics
IdentifiersURN: urn:nbn:se:uu:diva-163033DOI: 10.3109/17435390.2011.598245ISI: 000307227300007OAI: oai:DiVA.org:uu-163033DiVA: diva2:462504