Biomarkers of disease and post-mortem changes: Heat stabilization, a necessary tool for measurement of protein regulation
2011 (English)In: Journal of Proteomics, ISSN 1874-3919, Vol. 75, no 1, 145-159 p.Article, review/survey (Refereed) Published
This review focuses on post sampling changes and how the Stabilizor system has been used to control this natural biological process and potential implications on cancer-specific biomarkers due to post sampling changes. Tissue sampling is a major traumatic event that can have drastic effects within a very short timeframe at the molecular level  resulting in loss of sample quality due to post-mortem changes. A heat-stabilization technology, using the Stabilizor system, has been developed to quickly and permanently abolish the enzymatic activity that causes these changes post-sampling and so preserve sample quality. The Stabilizor system has been shown to give better sample quality when analyzing a variety of tissues in various proteomic workflows. In this paper we discuss the impact of using heat-stabilized tissue in different proteomic applications. Based on our observations regarding the overlap between commonly changing proteins and proteins found to change post-mortem we also highlight a group of proteins of particular interest in cancer studies.
Place, publisher, year, edition, pages
2011. Vol. 75, no 1, 145-159 p.
Other Natural Sciences
IdentifiersURN: urn:nbn:se:uu:diva-163496DOI: 10.1016/j.jprot.2011.06.009ISI: 000298710400015PubMedID: 21708298OAI: oai:DiVA.org:uu-163496DiVA: diva2:464174
Första-, andra- och tredjeförfattare delar författarskapet.2011-12-122011-12-122013-01-16Bibliographically approved