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Capsid Conformational Sampling in HK97 Maturation Visualized by X-Ray Crystallography and Cryo-EM
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2006 (English)In: Structure, ISSN 0969-2126, E-ISSN 1878-4186, Vol. 14, no 11, 1655-1665 p.Article in journal (Refereed) Published
Abstract [en]

Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II) to the mature state (Head II) involves a 60 angstrom radial expansion. The mature particle is formed by 420 copies of the major capsid protein organized on a T = 7 laevo, lattice with each subunit covalently crosslinked to two neighbors. Well-characterized pH 4 expansion intermediates make HK97 valuable for investigating quaternary structural dynamics. Here, we use X-ray crystallography and cryo-EM to demonstrate that in the final transition in maturation (requiring neutral pH), pentons in Expansion Intermediate IV (EI-IV) reversibly sample 14 angstrom translations and 6 degrees rotations relative to a fixed hexon lattice. The limit of this trajectory corresponds to the Head II conformation that is secured at this extent only by the formation of the final class of covalent crosslinks. Mutants that cannot crosslink or EI-IV particles that have been rendered incapable of forming the final crosslink remain in the EI-IV state.

Place, publisher, year, edition, pages
2006. Vol. 14, no 11, 1655-1665 p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-18888DOI: 10.1016/j.str.2006.09.006ISI: 000242127800009PubMedID: 17098191OAI: oai:DiVA.org:uu-18888DiVA: diva2:46660
Available from: 2006-11-23 Created: 2006-11-23 Last updated: 2011-05-10Bibliographically approved

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