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Trigger factor binding to ribosomes with nascent peptide chains of varying lengths and sequences
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2006 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 281, no 38, 28033-28038 p.Article in journal (Refereed) Published
Abstract [en]

Trigger factor (TF) is the first protein-folding chaperone to interact with a nascent peptide chain as it emerges from the ribosome. Here, we have used a spin down assay to estimate the affinities for the binding of TF to ribosome nascent chain complexes (RNCs) with peptides of varying lengths and sequences. An in vitro system for protein synthesis assembled from purified Escherichia coli components was used to produce RNCs stalled on truncated mRNAs. The affinity of TF to RNCs exposing RNA polymerase sequences increased with the length of the nascent peptides. TF bound to RNA polymerase RNCs with significantly higher affinity than to inner membrane protein leader peptidase and bacterioopsin RNCs. The latter two RNCs are substrates for signal recognition particle, suggesting complementary affinities of TF and signal recognition particle to nascent peptides targeted for cytoplasm and membrane.

Place, publisher, year, edition, pages
2006. Vol. 281, no 38, 28033-28038 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-18895DOI: 10.1074/jbc.M605753200ISI: 000240534400040PubMedID: 16829677OAI: oai:DiVA.org:uu-18895DiVA: diva2:46667
Available from: 2006-11-23 Created: 2006-11-23 Last updated: 2017-12-08Bibliographically approved

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Lovmar, MartinWikberg, JarlEhrenberg, Måns

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CiteExportLink to record
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