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In vitro and in vivo toxicity of rinsed and aged nanocellulose-polypyrrole composites
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0002-5496-9664
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences. (Renal Medicine)
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
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2012 (English)In: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, E-ISSN 1552-4965, Vol. 100A, no 8, 2128-2138 p.Article in journal (Refereed) Published
Abstract [en]

Novel composites of nanocellulose and the conducting polymer polypyrrole (PPy) are herein suggested as potential candidates for active ion-extraction membranes in electrochemically controlled hemodialysis. This work has defined processing parameters to obtain a biocompatible nanocellulose-PPy composite and for the first time, the effect of the composite ageing on cell viability has been studied.

The influence of rinsing and extraction process steps, as well as ageing under different conditions (i.e. in air, at –20 ˚C and in argon), on the electroactivity and cytotoxicity of a PPy-nanocellulose composite has been investigated. The biocompatibility evaluation was based on indirect toxicity assays with fibroblasts and monocyte cell lines and an acute toxicity test in mice, while the electroactivity was evaluated by cyclic voltammetry experiments.

The as-prepared composite did not induce any cytotoxic response in vitro or in vivo. Extensive rinsing and 48 hour incubation in biological buffer previous to the preparation of the culture medium extracts were, however, necessary to obtain a non-cytotoxic composite. The as-prepared composite was also found to exhibit acceptable electrochemical performance, which was retained upon 4 weeks storage in argon atmosphere.  It was shown that ageing of the composite had a negative effect on biocompatibility, regardless of the storage condition. Thus, to allow for long time storage of electroactive nanocellulose-PPy hemodialysis membranes, the degradation of PPy upon storage must be controlled. The present results show that the biocompatibility of PPy composites depends on the rinsing and pre-treatment of the composite material as well as the aging of the material.

Place, publisher, year, edition, pages
2012. Vol. 100A, no 8, 2128-2138 p.
Keyword [en]
polypyrrole, nanocellulose, cytotoxicity, hemodialysis, ageing
National Category
Medical Materials Inorganic Chemistry Nano Technology
Research subject
Chemistry with specialization in Inorganic Chemistry; Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-165375DOI: 10.1002/jbm.a.34070ISI: 000305509700023OAI: oai:DiVA.org:uu-165375DiVA: diva2:473192
Available from: 2012-01-05 Created: 2012-01-05 Last updated: 2017-12-08Bibliographically approved

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Ferraz, NataliaStrømme, MariaFellström, BengtNyholm, LeifMihranyan, Albert

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Ferraz, NataliaStrømme, MariaFellström, BengtNyholm, LeifMihranyan, Albert
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Nanotechnology and Functional MaterialsDepartment of Medical SciencesInorganic Chemistry
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Journal of Biomedical Materials Research. Part A
Medical MaterialsInorganic ChemistryNano Technology

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