Scanning near-field IR microscopy of proteins in lipid bilayers
2011 (English)In: Physical Chemistry, Chemical Physics - PCCP, ISSN 1463-9076, E-ISSN 1463-9084, Vol. 13, no 48, 21432-21436 p.Article in journal (Refereed) Published
We use infrared near-field microscopy to chemically map the morphology of biological matrices. The investigated sample is built up from surface-tethered membrane proteins (cytochrome c oxidase) reconstituted in a lipid bilayer. We have carried out infrared near-field measurements in the frequency range between 1600 and 1800 cm(-1). By simultaneously recording the topography and chemical fingerprint of the protein-tethered lipid bilayer with a lateral resolution of 80 nm x 80 nm, we were able to probe locally the chemical signature of this membrane and to provide a local map of its surface morphology.
Place, publisher, year, edition, pages
2011. Vol. 13, no 48, 21432-21436 p.
IdentifiersURN: urn:nbn:se:uu:diva-166103DOI: 10.1039/c1cp21512dISI: 000297560200029OAI: oai:DiVA.org:uu-166103DiVA: diva2:475586