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Quantitative subproteomic analysis of age-related changes in mouse liver peroxisomes by iTRAQ LC-MS/MS
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry.
2011 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 879, no 30, 3393-3400 p.Article in journal (Refereed) Published
Abstract [en]

Aging is a complex multifactorial phenomenon, which is believed to result from the accumulation of cellular damage to biological macromolecules. Peroxisomes recently emerged as another important source of reactive oxygen species (ROS) production in addition to mitochondria. However, the role of these organelles in the process of aging is still not clear. The aim of this study was to characterize the changes in protein expression profiles of young (10 weeks old) versus old (18 months old) mouse liver peroxisome-enriched fractions. We have applied shotgun proteomic approach based on liquid chromatography and tandem mass spectrometry (LC-MS/MS) combined with iTRAQ (isobaric tags for relative and absolute quantitation) labeling that allows comparative quantitative multiplex analysis. Our analysis led to identification and quantification of 150 proteins, 8 out of which were differentially expressed between two age groups at a statistically significant level (p < 0.05), with folds ranging from 1.2 to 4.1. These proteins involved in peroxisornal beta-oxidation, detoxification of xenobiotics and production of ROS. Noteworthy, differences in liver proteome have been observed between as well as within different age groups. In conclusion, our subproteomic quantitative study suggests that mouse liver proteome is sufficiently maintained until certain age.

Place, publisher, year, edition, pages
2011. Vol. 879, no 30, 3393-3400 p.
Keyword [en]
Liquid chromatography, Tandem mass spectrometry, Peroxisomes, Aging, Liver, iTRAQ
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-166066DOI: 10.1016/j.jchromb.2011.08.044ISI: 000297396200006OAI: oai:DiVA.org:uu-166066DiVA: diva2:476928
Available from: 2012-01-12 Created: 2012-01-10 Last updated: 2017-12-08Bibliographically approved

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Sjödin, Marcus O.D.Bergquist, Jonas

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