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Structural basis for CRISPR RNA-guided DNA recognition by Cascade
Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, The Netherlands.
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2011 (English)In: Nature Structural & Molecular Biology, ISSN 1545-9993, E-ISSN 1545-9985, Vol. 18, no 5, 529-536 p.Article in journal (Refereed) Published
Abstract [en]

The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity depends on a ribonucleoprotein complex called Cascade. Here we present the composition and low-resolution structure of Cascade and show how it recognizes double-stranded DNA (dsDNA) targets in a sequence-specific manner. Cascade is a 405-kDa complex comprising five functionally essential CRISPR-associated (Cas) proteins (CasA1B2C6D1E1) and a 61-nucleotide CRISPR RNA (crRNA) with 5′-hydroxyl and 2′,3′-cyclic phosphate termini. The crRNA guides Cascade to dsDNA target sequences by forming base pairs with the complementary DNA strand while displacing the noncomplementary strand to form an R-loop. Cascade recognizes target DNA without consuming ATP, which suggests that continuous invader DNA surveillance takes place without energy investment. The structure of Cascade shows an unusual seahorse shape that undergoes conformational changes when it binds target DNA.

Place, publisher, year, edition, pages
2011. Vol. 18, no 5, 529-536 p.
National Category
Biochemistry and Molecular Biology
Research subject
Biology with specialization in Microbiology
URN: urn:nbn:se:uu:diva-166885DOI: 10.1038/nsmb.2019PubMedID: 21460843OAI: oai:DiVA.org:uu-166885DiVA: diva2:478537
Available from: 2012-01-16 Created: 2012-01-16 Last updated: 2012-03-08Bibliographically approved

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