Independent thesis Advanced level (professional degree), 20 credits / 30 HE credits
Investigating the aerodynamic properties of mannitol-based multi-component dry powder carrier platforms for pulmonary delivery of influenza vaccine
Dr Michelle P. McIntosh, Department of Drug Delivery, Disposition and Dynamics.
Monash Institute of Pharmaceutical Sciences 30 hp. Margareta Hammarlund-Udenaes.
Introduction: Pulmonary delivery of influenza vaccine could be a new administration route that could improve safety and compliance, lower the medical costs and induce both systemic and mucosal immunity. Formulating the vaccine as an amorphous dry powder could keep the vaccine stable. To optimize the aerodynamic properties of the dry powder the influence of different excipients were investigated.
Aim: The aim of this study was to investigate the influence of leucine, glycine, alanine and trehalose on the aerodynamic properties of spray dried mannitol-based multi-component dry powder formulations for pulmonary delivery of influenza vaccine.
Materials and Methods: Different formulations consisting of mannitol, leucine and/or glycine/alanine/trehalose in different proportions were prepared in a powder form by spray drying. SEM was used to investigate particle morphology and size. The aerodynamic properties, size and lung deposition behavior in vitro were investigated by using NGI.
Results: The results showed that powders containing mannitol, leucine and glycine or alanine produced comparable fine powder fractions to powders of mannitol and leucine. Powders containing trehalose produces low fine powder fractions.
Conclusions: Powder formulations containing trehalose were too cohesive to produce reasonable fine powder fractions. Larger fine powder fractions were obtained when a higher fraction of leucine is present in the formulation. Powders containing glycine produced the largest fine powder fractions and powders containing alanine produced the largest fractions of particles in the range of 0.94-4.46 μm.
2012. , 30 p.