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Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2012 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 422, no 1, 46-58 p.Article in journal (Refereed) Published
Abstract [en]

Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2).

Place, publisher, year, edition, pages
2012. Vol. 422, no 1, 46-58 p.
Keyword [en]
Influenza, Spanish flu, NS1, Splicing, Polyadenylation, SR proteins, ASF/SF2, SRp30c, SRp40
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-165594DOI: 10.1016/j.virol.2011.10.004ISI: 000297902300006OAI: oai:DiVA.org:uu-165594DiVA: diva2:481848
Available from: 2012-01-23 Created: 2012-01-09 Last updated: 2017-12-08Bibliographically approved

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Lindström, SofiaTingsborg, SusanneSchwartz, Stefan

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