Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein
2012 (English)In: Virology, ISSN 0042-6822, E-ISSN 1096-0341, Vol. 422, no 1, 46-58 p.Article in journal (Refereed) Published
Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2).
Place, publisher, year, edition, pages
2012. Vol. 422, no 1, 46-58 p.
Influenza, Spanish flu, NS1, Splicing, Polyadenylation, SR proteins, ASF/SF2, SRp30c, SRp40
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-165594DOI: 10.1016/j.virol.2011.10.004ISI: 000297902300006OAI: oai:DiVA.org:uu-165594DiVA: diva2:481848