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Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular biophysics.
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2012 (English)In: Nature Photonics, ISSN 1749-4885, Vol. 6, no 1, 35-40 p.Article in journal (Refereed) Published
Abstract [en]

X-ray free-electron lasers have enabled new approaches to the structural determination of protein crystals that are too small or radiation-sensitive for conventional analysis(1). For sufficiently short pulses, diffraction is collected before significant changes occur to the sample, and it has been predicted that pulses as short as 10 fs may be required to acquire atomic-resolution structural information(1-4). Here, we describe a mechanism unique to ultrafast, ultra-intense X-ray experiments that allows structural information to be collected from crystalline samples using high radiation doses without the requirement for the pulse to terminate before the onset of sample damage. Instead, the diffracted X-rays are gated by a rapid loss of crystalline periodicity, producing apparent pulse lengths significantly shorter than the duration of the incident pulse. The shortest apparent pulse lengths occur at the highest resolution, and our measurements indicate that current X-ray free-electron laser technology(5) should enable structural determination from submicrometre protein crystals with atomic resolution.

Place, publisher, year, edition, pages
2012. Vol. 6, no 1, 35-40 p.
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Natural Sciences
URN: urn:nbn:se:uu:diva-167165DOI: 10.1038/NPHOTON.2011.297ISI: 000298416200013OAI: oai:DiVA.org:uu-167165DiVA: diva2:483469
Available from: 2012-01-25 Created: 2012-01-23 Last updated: 2016-04-12Bibliographically approved

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Caleman, CarlTimneanu, NicusorAndreasson, JakobDavidsson, JanEkeberg, TomasHajdu, JanosMaia, Filipe R. N. C.Seibert, M. Marvin
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