Kidney function after in vivo gene silencing of Uncoupling Protein-2 in streptozotocin-induced diabetic rats
2013 (English)In: Oxygen Transport to Tissue XXXIV, Springer-Verlag New York, 2013, Vol. 765, 217-223 p.Chapter in book (Refereed)
Kidney uncoupling protein 2 (UCP-2) increases in streptozotocin-induced diabetes, resulting in mitochondria uncoupling, i.e., increased oxygen consumption unrelated to active transport. The present study aimed to investigate the role of UCP-2 for normal and diabetic kidney function utilizing small interference RNA (siRNA) to reduce protein expression. Diabetic animals had increased glomerular filtration rate and kidney oxygen consumption, resulting in decreased oxygen tension and transported sodium per consumed oxygen. UCP-2 protein levels decreased 2 and 50% after UCP-2 siRNA administration in control and diabetic animals respectively. Kidney function was unaffected by in vivo siRNA-mediated gene silencing of UCP-2. The reason for the lack of effect of reducing UCP-2 is presently unknown but may involve compensatory mitochondrial uncoupling by the adenosine nucleotide transporter.
Place, publisher, year, edition, pages
Springer-Verlag New York, 2013. Vol. 765, 217-223 p.
, Advances in Experimental Medicine and Biology, ISSN 0065-2598 ; 765
IdentifiersURN: urn:nbn:se:uu:diva-167783DOI: 10.1007/978-1-4614-4989-8_30ISI: 000339280100031ISBN: 978-1-4614-4771-9ISBN: 978-1-4614-4989-8OAI: oai:DiVA.org:uu-167783DiVA: diva2:488017